SC35MH KT RRRR GLF
SC35MH-E KT - - - V GLF
N
C
v carries a
valine instead of a polybasic
cleavage site.
Elastase
No Protease
Trypsin
No Protease
SC35MH-E
SC35MH
v is
strictly elastase-dependent.
Hours
Viral
titer [Log10(pfu/ml)]
SC35MH without protease
SC35MH-E with elastase
v and grows to
similar high titers on MDCK
and Vero cells like the
wild-type.